Coding

Part:BBa_K1796011:Design

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)


nifN promoted from Paenibacillus sp. WLY78


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
    Illegal NgoMIV site found at 648
    Illegal AgeI site found at 25
    Illegal AgeI site found at 147
    Illegal AgeI site found at 766
    Illegal AgeI site found at 987
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut sites were involved(three PstI) after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.


Source

NifN is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.

References